special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.

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Cleavage of the polysaccharide chains causes agar softening and allows faster evaporation of water, leading to the formation of depressions. At longer incubation periods the level of agarase decreases, a trend probably due to the presence of proteases.

Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi

The protein was off batchwise with 90 ml of 1. Duckworth M, Turvey J R. The molecular mass of the enzyme was estimated by gel filtration by using Sephadex G25 and Superdex 75 columns.

An extracellular agarase was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography on DEAE-cellulose.

Manual of methods for general microbiology. The sequence of the 16S rDNA of strain Identirication was aligned with the sequences of a number of Pseudoalteromonas strains available and was analyzed essentially as described by Gauthier et al. Effect of carbon sources on bacterial growth and agarase production. Based in these data we propose the assignment of our strain as P.


The enzyme was stable under the conditions of iventification assay as determined by measuring the residual activity at pH 7. Based on this property, strain N-1 could be assigned to the genera Alteromonas 671820 or Pseudoalteromonas The purified agrolytic was determined to be homogeneous on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it had a molecular mass of 33 kDa.

Characterization of the neoagarotetraose and neoagarobiase of Cytophaga flevensis.

The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the bacferia Pacific coast was investigated. Effects of pH and temperature on enzyme activity. In our laboratory, we have isolated a few agar-softening and agar-liquefying bacterial strains from the southern Chilean coast to characterize their extracellular agarases in an attempt to contribute to our understanding of the iedntification of agar hydrolysis.

B Effect of temperature on the stability of agarase from P.

Genomic DNA was prepared by the procedure of Ausubel et al. Determination of carbohydrate metabolism of marine bacteria.

Some of the bacterial isolates have been assigned to the genera Alteromonas 12212733Cytophaga 43Streptomyces 36Vibrio 339and Pseudomonas Purification and properties of an extracellular agarase from Alteromonas sp. Previous results on the purification and characterization of an extracellular agarase from the agar-liquefying strain Alteromonas sp.

Strain N-1 is a gram-negative rod bacterium, motile by a polar flagellum; it is also obligate aerobic, catalase and oxidase positive, and urease, indole, and arginine dihydrolase negative. Nature London ; Nonpathogenic members of the genus Pseudomonas. Strain N-1 was isolated from decomposing algae in Niebla Valdivia, Chile. Agar clearing, softening, and depressions around the colonies is characteristic for bacteria in groups 1 and 2.


In contrast to the agarases from P. Phylogenetic analysis and assessment of the genera VibrioPhotobacteriumand Plesiomonas deduced from small-subunit rRNA sequences. The strain was gram negative, obligately aerobic, and polarly flagellated.

Purification and some properties of agarase from Pseudomonas sp.

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However, we must point out that strain N-1 differs from the type strain of this species in some properties. Further studies on the characterization of new agarases and their coding genes will be required to determine the significance of these conserved regions.

Enzymatic hydrolysis of agar: Hydrolysis products of agar by agarase from P. The screening was carried out on agar plates in a medium containing 0. The amount of pigment was dependent on the addition of tyrosine to the culture medium, suggesting the presence of a melaninlike pigment 2. The specificity of an agarase from a Cytophaga species.

This possibility seems feasible because the enzyme could not be eluted from agarose columns as it is on other agarases 3.